Anticorps Polyclonal de lapin anti-GABPB1

• Phare
• Validé par KD/KO

GABPB1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 12597-1-AP

Synonymes

BABPB2, E4TF1, E4TF1 47, E4TF1 53, E4TF1B, GABP subunit beta 1, GABP subunit beta 2, GABPB, GABPB 1, GABPB 2, GABPB1, GABPB2, NRF2B1, NRF2B2, Nuclear respiratory factor 2, Transcription factor E4TF1 47, Transcription factor E4TF1 53

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Galerie de données de validation

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  WB analysis using 12597-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12597-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12597-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12597-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12597-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12597-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12597-1-AP

  IP Result of anti-GABPB1 (IP:12597-1-AP, 4ug; Detection:12597-1-AP 1:1000) with HeLa cells lysate 520ug.

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HeLa using 12597-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GABPB1 antibody (12597-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, tissu pulmonaire de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12597-1-AP cible GABPB1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GABPB1 Protéine recombinante Ag3208
• Nom complet: GA binding protein transcription factor, beta subunit 1
• Masse moléculaire calculée: 347 aa, 38 kDa
• Poids moléculaire observé: 36-45 kDa, 47-53 kDa
• Numéro d’acquisition GenBank: BC016910
• Symbole du gène: GABPB1
• Identification du gène (NCBI): 2553
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GABPB1, also named as E4TF1B, GABPB, GABPB2, E4TF1-47 and E4TF1-53, is a transcription factor capable of interacting with purine rich repeats (GA repeats). It is necessary for the expression of the Adenovirus E4 gene. GABPB1 has 4 isoforms. The MW-of Beta 1 is 53kd and Beta-2 is 47kd(PMID:9525935). This antibody can recognize all the isoforms.

Publications

Species	Application	Title
human	WB	Cancer Cell; Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner.; Authors - Andrew Mancini ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage.; Authors - Alexandra M Amen
human, mouse	WB,IHC,IF	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H ; KD Validated
human	WB	Sci Rep; LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells.; Authors - Wenchuan Qi ; KD Validated
human	WB	Biology (Basel); TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.; Authors - Yanbing Wang
mouse	WB	Brain Res Bull; ZFP36 protects against oxygen-glucose deprivation/reoxygenation-induced mitochondrial fragmentation and neuronal apoptosis through inhibiting NOX4-DRP1 pathway.; Authors - Hengjiang Guo