Anticorps Polyclonal de lapin anti-GABRA1

GABRA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(41)

Avis (1)

N° de cat : 12410-1-AP

Synonymes

ECA4, EJM, GABA A receptor alpha 1, GABAA receptor alpha 1, GABRA1

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Galerie de données de validation

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  WB analysis using 12410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12410-1-AP (GABRA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12410-1-AP

  IP result of anti-GABRA1 (IP:12410-1-AP, 3ug; Detection:12410-1-AP 1:400) with mouse brain tissue lysate 3600ug.

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  IHC staining of rat brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12410-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12410-1-AP (GABRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 32 publications below
• IHC: See 2 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

12410-1-AP cible GABRA1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GABRA1 Protéine recombinante Ag3117
• Nom complet: gamma-aminobutyric acid (GABA) A receptor, alpha 1
• Masse moléculaire calculée: 456 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC030696
• Symbole du gène: GABRA1
• Identification du gène (NCBI): 2554
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse	IF	Adv Mater; Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment; Authors - Yuewen Zhai
mouse	WB	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang
human		Neurol Neuroimmunol Neuroinflamm; MRI Characteristics of Autoimmune Encephalitis With Autoantibodies to GABAA Receptor: A Case Series.; Authors - Bo Deng
mouse	WB	Mol Ther Nucleic Acids; Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.; Authors - Colleen S Stein
mouse	WB	Int J Mol Sci; The Effect of TGF-β1 Reduced Functionality on the Expression of Selected Synaptic Proteins and Electrophysiological Parameters: Implications of Changes Observed in Acute Hepatic Encephalopathy.; Authors - Mariusz Popek

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (11-05-2024)

This gabra1 antibody works perfectly, the best I have tried so far. It shows perfectly the puncta on the axons of gabaergic neurons on the upper layers of the cortex.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

GABRA1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:12410-1-AP)

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