Anticorps Recombinant de lapin anti-GABRA2

GABRA2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

230158E6

N° de cat : 83057-2-RR

Synonymes

230158E6

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Galerie de données de validation

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  WB analysis using 83057-2-RR

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83057-2-RR (GABRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83057-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83057-2-RR (GABRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83057-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GABRA2 Recombinant antibody (83057-2-RR, Clone:230158E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83057-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83057-2-RR against Human GABRA2 were performed. The affinity constant is 0.403 nM.

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

83057-2-RR cible GABRA2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GABRA2 Protéine recombinante Ag29301
• Nom complet: gamma-aminobutyric acid (GABA) A receptor, alpha 2
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-51 kDa
• Numéro d’acquisition GenBank: NM_000807
• Symbole du gène: GABRA2
• Identification du gène (NCBI): 2555
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; Flavonoids from mulberry leaves exhibit sleep-improving effects via regulating GABA and 5-HT receptors; Authors - Rui Li
rat	WB	Int Immunopharmacol; Promotion of sleep by cinnamic acid in parachlorophenylalanine-induced insomnia in rats; Authors - Xiaona Ye

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