Anticorps Recombinant de lapin anti-GABRA2

GABRA2 Recombinant Antibody for WB, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra)

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230158E6

N° de cat : CL750-83057-2

Synonymes

230158E6

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Galerie de données de validation

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  WB analysis using CL750-83057-2

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83057-2 (GABRA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using CL750-83057-2

  1x10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated GABRA2 Recombinant antibody (CL750-83057-2, Clone:230158E6)(red), or 4 ug Isotype Control (CL750-30000, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: unboiled rat brain tissue, tissu cérébral de souris non bouilli, tissu de cervelet de souris
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-83057-2 cible GABRA2 dans les applications de WB, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GABRA2 Protéine recombinante Ag29301
• Nom complet: gamma-aminobutyric acid (GABA) A receptor, alpha 2
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-51 kDa
• Numéro d’acquisition GenBank: NM_000807
• Symbole du gène: GABRA2
• Identification du gène (NCBI): 2555
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.

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