Anticorps Polyclonal de lapin anti-Galectin-1

• Phare
• Validé par KD/KO

Galectin-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(22)

N° de cat : 11858-1-AP

Synonymes

Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1, Galaptin

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 11858-1-AP

  WB result of Galectin-1 antibody (11858-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin-1 transfected HepG2 cells.

• 

  WB analysis using 11858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 11858-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Galectin-1 antibody (11858-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HCT 116, cellules HepG2, cellules MCF-7, cellules NIH/3T3, cellules PC-12, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IHC: See 8 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

11858-1-AP cible Galectin-1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Galectin-1 Protéine recombinante Ag2432
• Nom complet: lectin, galactoside-binding, soluble, 1
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC020675
• Symbole du gène: Galectin-1
• Identification du gène (NCBI): 3956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Publications

Species	Application	Title
mouse,human	WB,IF	Dev Cell; Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption; Authors - Mobai Li
human	IF	Front Med; Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis; Authors - Weiwei Xian
human	WB	Cell Rep; Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.; Authors - Krishna S Tummala
human	WB	Mol Ther Nucleic Acids; Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.; Authors - Chen Yang
human	WB,IHC	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
mouse	IHC	Biomed Pharmacother; Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction.; Authors - Yoojin Seo

{{ptg:RelatedPrimaryAntibodies}}