Anticorps Polyclonal de lapin anti-GAPDHS

GAPDHS Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 13937-1-AP

Synonymes

GAPD2, GAPDH 2, GAPDH2, GAPDHS, GAPDS, HSD 35

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Galerie de données de validation

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  WB analysis using 13937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13937-1-AP (GAPDHS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13937-1-AP (GAPDHS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below

Informations sur le produit

13937-1-AP cible GAPDHS dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GAPDHS Protéine recombinante Ag4988
• Nom complet: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC036373
• Symbole du gène: GAPDHS
• Identification du gène (NCBI): 26330
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, testis-specific) is also named as GAPD2, GAPDH2, GAPDS and belongs to the glyceraldehyde-3-phosphate dehydrogenase family. It may play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. GAPDHS is a promising contraceptive target because it is specific to male germ cells, essential for sperm motility and male fertility, and well suited to pharmacological inhibition(PMID:20828617).

Publications

Species	Application	Title
mouse	WB	J Hazard Mater; Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.; Authors - Zijun Peng
human	WB	Theranostics; Phosphorylation of IRS4 by CK1γ2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway.; Authors - Xinchun Li
human	WB	Theranostics; Up-regulation of PCOLCE by TWIST1 promotes metastasis in Osteosarcoma.; Authors - Shang Wang
mouse	WB	Oxid Med Cell Longev; Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner.; Authors - Jingya Gu
rat	WB	J Cell Mol Med; Celastrol exerts anti-inflammatory effect in liver fibrosis via activation of AMPK-SIRT3 signalling.; Authors - Yuqin Wang
human	WB	Front Cell Dev Biol; Sperm-Specific Glycolysis Enzyme Glyceraldehyde-3-Phosphate Dehydrogenase Regulated by Transcription Factor SOX10 to Promote Uveal Melanoma Tumorigenesis.; Authors - Xia Ding

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