Anticorps Polyclonal de lapin anti-GATA6

• Phare
• Validé par KD/KO

GATA6 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 55435-1-AP

Synonymes

GATA binding factor 6, GATA binding protein 6, GATA6, Transcription factor GATA 6

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Galerie de données de validation

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  WB analysis of MCF-7 using 55435-1-AP

  WB result of GATA6 antibody (55435-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA6 transfected MCF-7 cells.

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  WB analysis using 55435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55435-1-AP (GATA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 55435-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55435-1-AP (GATA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 55435-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HuH-7 using 55435-1-AP

  1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human GATA6 (55435-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules BxPC-3, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu hépatique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HuH-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

55435-1-AP cible GATA6 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GATA binding protein 6
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60-64 kDa
• Numéro d’acquisition GenBank: NM_005257
• Symbole du gène: GATA6
• Identification du gène (NCBI): 2627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GATA6 is thought to be important for regulating terminal differentiation and/or proliferation.

Publications

Species	Application	Title
mouse	WB	Environ Sci Technol; Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling.; Authors - Zhihua Ren
mouse	WB,IF	iScience; Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency; Authors - Qian Ban
human	WB	FASEB J; Pulmonary expression of CYP2A13 and ABCB1 is regulated by FOXA2 and their genetic interaction is associated with lung cancer.; Authors - Chan Xiang
mouse	WB	Front Cell Dev Biol; ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4.; Authors - XiaoYun Sun
human	WB	Cell Rep; METTL14 regulates chromatin bivalent domains in mouse embryonic stem cells; Authors - Mandi Mu
human	WB	Hum Cell; MicroRNA-10a-5p-mediated downregulation of GATA6 inhibits tumor progression in ovarian cancer; Authors - Feiying Gao