Anticorps Polyclonal de lapin anti-GATAD2B

GATAD2B Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 25679-1-AP

Synonymes

Transcriptional repressor p66-beta, P66beta, p66/p68, GATA zinc finger domain-containing protein 2B

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Galerie de données de validation

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  WB analysis of A549 using 25679-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25679-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25679-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25679-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25679-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25679-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 25679-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using GATAD2B antibody (25679-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules K-562, cellules MCF-7, cellules PC-3
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

25679-1-AP cible GATAD2B dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GATAD2B Protéine recombinante Ag22615
• Nom complet: GATA zinc finger domain containing 2B
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC069419
• Symbole du gène: GATAD2B
• Identification du gène (NCBI): 57459
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
mouse	WB,IP	Cell Prolif; GATAD2B is required for pre-implantation embryonic development by regulating zygotic genome activation; Authors - Yuling Lin
human	WB	Nucleic Acids Res; CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development; Authors - Xue Chen

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