Anticorps Polyclonal de lapin anti-GATM

GATM Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 12801-1-AP

Synonymes

AGAT, AT, GATM, Transamidinase

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Galerie de données de validation

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  WB analysis of mouse kidney using 12801-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12801-1-AP (GATM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 12801-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12801-1-AP (GATM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12801-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12801-1-AP (GATM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 12801-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12801-1-AP (GATM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12801-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12801-1-AP (GATM antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu pancréatique de rat, tissu rénal de rat, tissu rénal humain
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

12801-1-AP cible GATM dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GATM Protéine recombinante Ag3754
• Nom complet: glycine amidinotransferase (L-arginine:glycine amidinotransferase)
• Masse moléculaire calculée: 423 aa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC004141
• Symbole du gène: GATM
• Identification du gène (NCBI): 2628
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.

Publications

Species	Application	Title
mouse	WB,IHC	Biomed Pharmacother; Metabolomic profiling of metoprolol-induced cardioprotection in a murine model of acute myocardial ischemia.; Authors - Qiong Lai
mouse	WB	J Agric Food Chem; Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.; Authors - Sho Nishikawa
mouse	WB	Front Physiol; Arginine:Glycine Amidinotransferase Is Essential for Creatine Supply in Mice During Chronic Hypoxia.; Authors - Juliane Hannemann
mouse	WB	Front Nutr; Influence of homoarginine on creatine accumulation and biosynthesis in the mouse; Authors - Craig A. Lygate
mouse	WB	Amino Acids; Muscle phenotype of AGAT- and GAMT-deficient mice after simvastatin exposure.; Authors - Ali Sasani
human	IF	Gene; Clinical and functional characterization of rare compound heterozygous mutations in the SERPINC1 gene causing severe thrombophilia; Authors - Ke Zhang