Anticorps Monoclonal anti-GBP1

GBP1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2A10E8

N° de cat : 67161-1-Ig

Synonymes

GBP 1, GBP1, GTP binding protein 1, HuGBP 1

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Galerie de données de validation

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  WB analysis of HeLa using 67161-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 67161-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67161-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67161-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 67161-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 67161-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 67161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HUVEC, tissu placentaire humain, tissu splénique de porc, tissu splénique de rat
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

67161-1-Ig cible GBP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GBP1 Protéine recombinante Ag7767
• Nom complet: GTP binding protein 1
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC002666
• Symbole du gène: GBP1
• Identification du gène (NCBI): 2633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II IFNs and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.

Publications

Species	Application	Title
human	WB,IF	Front Immunol; GBP1 Facilitates Indoleamine 2,3-Dioxygenase Extracellular Secretion to Promote the Malignant Progression of Lung Cancer.; Authors - Yinnan Meng
Chinese tree shrews	WB,IF	J Immunol; Tupaia GBP1 Interacts with STING to Initiate Autophagy and Restrict Herpes Simplex Virus Type 1 Infection.; Authors - Tianle Gu