Anticorps Polyclonal de lapin anti-GCC1

GCC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (2)

N° de cat : 16271-1-AP

Synonymes

GCC1, GCC1P, GCC88, Golgi coiled coil protein 1

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Galerie de données de validation

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  WB analysis using 16271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16271-1-AP (GCC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16271-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16271-1-AP (GCC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16271-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16271-1-AP (GCC1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16271-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16271-1-AP (GCC1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 16271-1-AP

  Immunofluorescent analysis of A431 cells using 16271-1-AP (GCC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu ovarien de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

16271-1-AP cible GCC1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GCC1 Protéine recombinante Ag9339
• Nom complet: GRIP and coiled-coil domain containing 1
• Masse moléculaire calculée: 775 aa, 88 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC014100
• Symbole du gène: GCC1
• Identification du gène (NCBI): 79571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GCC1 (Golgi coiled coil protein 1), also known as GCC1P or GCC88, is a protein probably involved in maintaining Golgi structure. The MW of this protein is 105 kDa, and Catalog#16271-1-AP specially recognises the 105 kDa protein.

Publications

Species	Application	Title
human	WB	J Biol Chem; GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.; Authors - Erpan Ahat
human	WB	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
human	WB	Mol Biol Cell; DjA1 maintains Golgi integrity via interaction with GRASP65.; Authors - Jie Li
human	WB	bioRxiv; SARS-CoV-2 triggers Golgi fragmentation via down-regulation of GRASP55 to facilitate viral trafficking.; Authors - Jianchao Zhang
human	WB,IF	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
	WB,IF	Methods Mol Biol; Common Markers and Small Molecule Inhibitors in Golgi Studies; Authors - Sarah Bui

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-28-2023)

clean band around the expected size

• Applications: Western Blot

FH

Jie (Verified Customer) (09-17-2020)

Works well for WB and IF with HeLa and CHO cells

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: HeLa, CHO

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