Anticorps Polyclonal de lapin anti-GCGR

GCGR Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 26784-1-AP

Synonymes

glucagon receptor, GL-R, GL R, GGR

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Galerie de données de validation

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  WB analysis of mouse liver using 26784-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26784-1-AP (GCGR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26784-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26784-1-AP (GCGR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skeletal muscle using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu cardiaque de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

26784-1-AP cible GCGR dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GCGR Protéine recombinante Ag24861
• Nom complet: glucagon receptor
• Masse moléculaire calculée: 477 aa, 54 kDa
• Poids moléculaire observé: 62-68 kDa
• Numéro d’acquisition GenBank: BC104854
• Symbole du gène: GCGR
• Identification du gène (NCBI): 2642
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucagon receptor (GCGR) is a secretin-like (class B) family of G-protein coupled receptors (GPCRs). It plays an important role in elevating the glucose concentration in the blood and has thus become one of the promising therapeutic targets for treating type 2 diabetes mellitus (PMID: 26236379). GCGR is a 62-kDa glycoprotein that contains at least four N-linked oligosaccharide chains (PMID: 2174441; 15245877). Defects in the gene of GCGR cause non-insulin-dependent diabetes mellitus (NIDDM).

Publications

Species	Application	Title
mouse	WB,IF	iScience; Pro-α-cell-derived β-cells contribute to β-cell neogenesis induced by antagonistic glucagon receptor antibody in type 2 diabetic mice.; Authors - Xiaona Cui
		J Gastroenterol Hepatol; Irisin Alleviates Impaired Mitochondrial Fusion via Enhancing PKA/SIRT3/mTOR Pathway in Hepatic Steatosis; Authors - Jia Li
mouse	WB	Mol Cell Endocrinol; Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.; Authors - Xinghong Guo
mouse	WB	Toxicology; Tributyltin exposure disturbs hepatic glucose metabolism in male mice.; Authors - Jing Xu
mouse	WB,IF	Am J Physiol Endocrinol Metab; Glucagon receptor blockage inhibits β-cell dedifferentiation through FoxO1; Authors - Kangli Wang
mouse	IHC,WB	Biomed Pharmacother; Si-Miao-Yong-An decoction preserves cardiac function and regulates GLC/AMPK/NF-κB and GLC/PPARα/PGC-1α pathways in diabetic mice; Authors - Lin Li

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