Anticorps Polyclonal de lapin anti-GDI1

GDI1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10249-1-AP

Synonymes

GDI 1, GDI1, GDIL, GDP dissociation inhibitor 1, MRX41, MRX48, Oligophrenin 2, OPHN2, Protein XAP 4, Rab GDI alpha, RABGD1A, RABGDIA, XAP 4, XAP4

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Galerie de données de validation

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  WB analysis of mouse brain using 10249-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10249-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10249-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10249-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10249-1-AP

  IP Result of anti-GDI1 (IP:10249-1-AP, 4ug; Detection:10249-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 4 publications below
• ELISA: See 1 publications below

Informations sur le produit

10249-1-AP cible GDI1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GDI1 Protéine recombinante Ag0288
• Nom complet: GDP dissociation inhibitor 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000317
• Symbole du gène: GDI1
• Identification du gène (NCBI): 2664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55kda protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034) This antibody can bind both GDIs for the close sequences.

Publications

Species	Application	Title
human	WB,IF	Neurology; Antibodies in patients with neuropsychiatric systemic lupus erythematosus.; Authors - Kimura A A
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
mouse	WB	Neurobiol Dis; Disruption of Rab11 activity in a knock-in mouse model of Huntington's disease.; Authors - Xueyi Li
human,mouse	ELISA	Proteomics; 2-D DIGE analysis implicates cytoskeletal abnormalities in psychiatric disease.; Authors - English Jane A JA
mouse	IF	Int J Mol Sci; Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage; Authors - Masato Mashimo
human	IF	Nature; A cross-species proteomic map reveals neoteny of human synapse development; Authors - Li Wang