Anticorps Polyclonal de lapin anti-GFI1

GFI1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 14198-1-AP

Synonymes

GFI 1, GFI1, Zinc finger protein 163, Zinc finger protein Gfi 1, ZNF163

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Galerie de données de validation

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  WB analysis of K-562 using 14198-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14198-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14198-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14198-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14198-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14198-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14198-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 14198-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14198-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HL-60, cellules PC-3, cellules Raji, RAW264.7, tissu de thymus de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

14198-1-AP cible GFI1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GFI1 Protéine recombinante Ag5401
• Nom complet: growth factor independent 1 transcription repressor
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 62-66 kDa
• Numéro d’acquisition GenBank: BC032751
• Symbole du gène: GFI1
• Identification du gène (NCBI): 2672
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GFI is a transcriptional repressor that is transiently induced during T-cell differentiation. It has critical roles in enhancing T helper-2 (Th2) cell expansion and in repressing induction of Th17 and CD103-positive inducible regulatory T (Treg) cells [PMID: 19188499]. GFI1 transcriptional regulator oncoprotein plays a crucial role in hematopoietic, inner ear, and pulmonary neuroendocrine cell development and governs cell processes as diverse as self-renewal of hematopoietic stem cells, proliferation, apoptosis, differentiation, cell fate specification, and oncogenesis (PMID: 18805794). The calculated molecular weight of GFI1 protein is about 45 kDa, but the modification of ubiquitinated GIF1 protein is about 62-66 kDa (PMID: 12721361).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda

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