Anticorps Polyclonal de lapin anti-GGPS1

• Phare
• Validé par KD/KO

GGPS1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 14944-1-AP

Synonymes

GGPP synthase, GGPPS, GGPPS1, GGPPSase, GGPS1

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Galerie de données de validation

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  WB analysis of mouse brain using 14944-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14944-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14944-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14944-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 14944-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14944-1-AP

  IP Result of anti-GGPS1 (IP:14944-1-AP, 4ug; Detection:14944-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human kidney using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14944-1-AP (GGPS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14944-1-AP (GGPS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14944-1-AP (GGPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14944-1-AP (GGPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14944-1-AP (GGPS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14944-1-AP (GGPS1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque humain, tissu placentaire humain, tissu testiculaire humain, tissu vésical de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

14944-1-AP cible GGPS1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GGPS1 Protéine recombinante Ag6745
• Nom complet: geranylgeranyl diphosphate synthase 1
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC067768
• Symbole du gène: GGPS1
• Identification du gène (NCBI): 9453
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GGPS1(Geranylgeranyl pyrophosphate synthase) belongs to the FPP/GGPP synthase family and catalyzes the synthesis of GGPP, an important molecule responsible for the C20-prenylation of protein and for the regulation of a nuclear hormone receptor. GGPP synthase puri¢ed from bovine brain is reported to be a homo-oligomer (150-195 kDa) with a molecular mass of 37.5 kDa for the monomer. This is consistent with the calculated molecular mass (35 kDa) of mouse and human GGPP synthases (PMID:10101267).

Publications

Species	Application	Title
mouse	IF	Dev Cell; Quantitative proteomic profiling identifies global protein network dynamics in murine embryonic heart development; Authors - Whitney Edwards
mouse	WB	FEBS J; Conditional Loss of Geranylgeranyl Diphosphate Synthase Alleviates Acute Obstructive Cholestatic Liver Injury by Regulating Hepatic Bile Acid Metabolism.; Authors - Wen-Jun Jia
	IHC	Am J Pathol; Geranylgeranyl Diphosphate Synthase Modulates Fetal Lung Branching Morphogenesis Possibly through Controlling K-Ras Prenylation.; Authors - Wen-Jun Jia
human	WB,IHC	J Cell Mol Med; Overexpression of geranylgeranyl diphosphate synthase contributes to tumour metastasis and correlates with poor prognosis of lung adenocarcinoma.; Authors - Xiaoxia Wang ; KD Validated
mouse	WB,IF	Cells; Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model.; Authors - Natalie Längst
human	WB	J Hepatocell Carcinoma; miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway; Authors - Kailin Xing