Anticorps Polyclonal de lapin anti-Growth Hormone

Growth Hormone Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 55243-1-AP

Synonymes

GH, GH1, growth hormone 1, GH-N, GH 1

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Galerie de données de validation

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  WB analysis of human placenta using 55243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55243-1-AP (GH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 55243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55243-1-AP (GH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 55243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55243-1-AP (GH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pituitary using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary adenoma using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pituitary adenoma using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain
• Résultats positifs en IHC: tissu hypophysaire humain, tissu d'adénome hypophysaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

55243-1-AP cible Growth Hormone dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GH1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: NM_000515
• Symbole du gène: GH1
• Identification du gène (NCBI): 2688
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. This antibody has no cross reaction to CSH1.

Publications

Species	Application	Title
rat	IF	Metabolism; PMID: 31666193; Authors - Jian Mao
mouse	WB	Psychoneuroendocrinology; Transcriptomics study of hippocampus in mice exposed to heat stress; Authors - Bin Li
mouse	WB,IF	Biochem Biophys Res Commun; Elongator regulates the melanocortin satiety pathway.; Authors - Joseph Walters
rat	WB	Toxicol Lett; The disrupting effect of chlormequat chloride on growth hormone is associated with pregnancy; Authors - Zongzhen Wu
	IF	Front Sleep; Regulation of dendritic spines in the amygdala following sleep deprivation; Authors - Lindsay Rexrode
human	WB	Talanta; What does one-dimensional gel electrophoresis-based western blotting data really mean in the reality of proteoforms; Authors - Lamei Yang

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