Anticorps Polyclonal de lapin anti-GHRL

GHRL Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13309-1-AP

Synonymes

Appetite regulating hormone, Ghrelin Obestatin, GHRL, Motilin related peptide, MTLRP, obestatin, Protein M46

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Galerie de données de validation

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  WB analysis of human stomach using 13309-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13309-1-AP (GHRL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 13309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 13309-1-AP (GHRL Antibody) at dilution of 1:400.

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  IHC staining of human stomach using 13309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 13309-1-AP (GHRL Antibody) at dilution of 1:400.

• 

  IHC staining of rat stomach using 13309-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach slide using 13309-1-AP (GHRL Antibody) at dilution of 1:50.

• 

  IHC staining of rat stomach using 13309-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach slide using 13309-1-AP (GHRL Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu d'estomac humain
• Résultats positifs en IHC: tissu d'estomac humain, tissu d'estomac de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

13309-1-AP cible GHRL dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GHRL Protéine recombinante Ag4141
• Nom complet: ghrelin/obestatin prepropeptide
• Masse moléculaire calculée: 117 aa, 13 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC025791
• Symbole du gène: GHRL
• Identification du gène (NCBI): 51738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is predominantly produced in endocrine cells in the gastric mucosa, called X/A-like or ghrelin cells, from where it is secreted into the plasma. In the pituitary gland, ghrelin stimulates GH release and regulates food intake and energy metabolism. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes.

Publications

Species	Application	Title
rat	WB	Phytomedicine; Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.; Authors - Ping Ma
rat	WB	Pancreas; Effects of Ghrelin miRNA on Inflammation and Calcium Pathway in Pancreatic Acinar Cells of Acute Pancreatitis; Authors -
human	IHC	Biosci Rep; Differential expression of ghrelin and GHSR via the mTOR pathway during the dynamic carcinogenic process involving oral, potentially malignant disorders.; Authors - Jianing Lou
mouse	IHC	Neuroreport; Expression of ghrelin or growth hormone secretagogue receptor in the brain of postpartum stress mice.; Authors - Jing-Wei Xing
rat	WB	Hepatobiliary Pancreat Dis Int; Ghrelin inhibits IKKβ/NF-κB activation and reduces pro-inflammatory cytokine production in pancreatic acinar AR42J cells treated with cerulein.; Authors - Ren-Jie Chang