Anticorps Polyclonal de lapin anti-GIPC1

• Phare
• Validé par KD/KO

GIPC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (2)

N° de cat : 14822-1-AP

Synonymes

C19orf3, GIPC, GIPC1, GLUT1CBP, Hs.6454, IIP 1, NIP, RGS GAIP interacting protein, RGS19 interacting protein 1, RGS19IP1, SEMCAP, SYNECTIIN, SYNECTIN, Tax interaction protein 2, TIP 2

---

Galerie de données de validation

• 

  WB analysis using 14822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14822-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14822-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14822-1-AP

  IP Result of anti-GIPC1 (IP:14822-1-AP, 4ug; Detection:14822-1-AP 1:500) with mouse brain tissue lysate 9500ug.

• 

  IHC staining of human breast cancer using 14822-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14822-1-AP (GIPC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14822-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14822-1-AP (GIPC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14822-1-AP

  Immunofluorescent analysis of HepG2 cells using 14822-1-AP (GIPC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A2780 using 14822-1-AP

  Immunofluorescent analysis of A2780 cells, using 14822-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules A2780

Dilution recommandée

• Western Blot (WB): WB : 1:600-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

14822-1-AP cible GIPC1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GIPC1 Protéine recombinante Ag6597
• Nom complet: GIPC PDZ domain containing family, member 1
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC000410
• Symbole du gène: GIPC1
• Identification du gène (NCBI): 10755
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Am J Hum Genet; Expansion of GGC Repeat in GIPC1 Is Associated with Oculopharyngodistal Myopathy.; Authors - Jianwen Deng
human	IHC	Dev Cell; SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt.; Authors - Christoph J Burckhardt
human	WB	Ann Neurol; GIPC1 CGG repeat expansion is associated with movement disorders.; Authors - Yu Fan
mouse	WB	Pain; G protein-coupled receptor GPR151 is involved in trigeminal neuropathic pain through the induction of Gβγ/extracellular signal-regulated kinase-mediated neuroinflammation in the trigeminal ganglion.; Authors - Bao-Chun Jiang
mouse	WB	FASEB J; BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.; Authors - Nahoko Eiraku ; KD Validated
human,mouse	WB	CNS Neurosci Ther; G-alpha interacting protein interacting protein, C terminus 1 regulates epileptogenesis by increasing the expression of metabotropic glutamate receptor 7.; Authors - Yong Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tom (Verified Customer) (11-02-2023)

Used at 1:2000 on PVDF membrane, overnight incubation at 4C in 3% BSA PBS 0.1% Tween. No non-specific bands and band reduced by knockdown with GIPC1 siRNA. Diluted antibody succesfully re0used after freezing at -20C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HUVEC

GIPC1 Antibody Western Blot validation (1:2000 dilution) in HUVEC (Cat no:14822-1-AP)

FH

Sophy (Verified Customer) (01-27-2023)

I ordered a GIPC1 antibody from another company before and the results were poor. Proteintech antibody worked very well with the dilution of 1:1000 in western.

• Applications: Western Blot
• Cell Tissue Type: NSCLC cells