Anticorps Polyclonal de lapin anti-GJB3

• Phare
• Validé par KD/KO

GJB3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 12880-1-AP

Synonymes

Connexin 31, CX31, DFNA2, DFNA2B, EKV, Gap junction beta 3 protein, GJB3

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Galerie de données de validation

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  WB analysis of HeLa using 12880-1-AP

  WB result of GJB3 antibody (12880-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GJB3 transfected HeLa cells.

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  WB analysis using 12880-1-AP

  mouse stomach tissue was subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 12880-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12880-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12880-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12880-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12880-1-AP

  IP result of anti-GJB3 (IP:12880-1-AP, 4ug; Detection:12880-1-AP 1:500) with HeLa cells lysate 2600 ug.

• 

  IHC staining of human cervix using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse placenta using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 12880-1-AP (GJB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 12880-1-AP (GJB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12880-1-AP (GJB3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 12880-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12880-1-AP (GJB3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu d'estomac de souris, cellules HeLa, tissu ovarien de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu placentaire de souris, tissu de cancer de la peau humain, tissu de col de l'utérus humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

12880-1-AP cible GJB3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GJB3 Protéine recombinante Ag3839
• Nom complet: gap junction protein, beta 3, 31kDa
• Masse moléculaire calculée: 270 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC012918
• Symbole du gène: GJB3
• Identification du gène (NCBI): 2707
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GJB3, also known as Connexin-31 (Cx31), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Mutations in the gene of GJB3 have been described in patients with dominant and recessive hearing impairment and in patients with erythrokeratodermia variabilis (EKV).

Publications

Species	Application	Title
human	WB	Cell Rep; Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss.; Authors - Makoto Hosoya
mouse	IHC	Stem Cells; FBXL12-Mediated Degradation of ALDH3 Is Essential for Trophoblast Differentiation during Placental Development.; Authors - Masaaki Nishiyama
mouse	IF	J Neurosci; Glial-Specific Deletion of Med12 Results in Rapid Hearing Loss via Degradation of the Stria Vascularis.; Authors - Teng-Wei Huang
marmoset	IF	Sci Rep; Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea.; Authors - Makoto Hosoya
mouse	IF	Placenta; In vitro fertilization causes excessive glycogen accumulation in mouse placenta.; Authors - Jie Dong
mouse	IHC	J Pain Res; PMID: 29692624; Authors - Brice Bellessort

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