Anticorps Polyclonal de lapin anti-Glycerokinase

Glycerokinase Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13360-1-AP

Synonymes

GK, GK1, GKD, Glycerokinase, glycerol kinase

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Galerie de données de validation

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  WB analysis of HepG2 using 13360-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13360-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13360-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13360-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13360-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13360-1-AP

  IP Result of anti-Glycerokinase (IP:13360-1-AP, 4ug; Detection:13360-1-AP 1:1000) with HepG2 cells lysate 4000ug.

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  IF Staining of HepG2 using 13360-1-AP

  Immunofluorescent analysis of HepG2 cells using 13360-1-AP (Glycerokinase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu hépatique de souris, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

13360-1-AP cible Glycerokinase dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glycerokinase Protéine recombinante Ag4175
• Nom complet: glycerol kinase
• Masse moléculaire calculée: 524 aa, 57 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC037549
• Symbole du gène: GK
• Identification du gène (NCBI): 2710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
pig	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu
mouse	WB	J Appl Toxicol; Aberrant expression of miR-451a contributes to 1,2-dichloroethane-induced hepatic glycerol gluconeogenesis disorder by inhibiting glycerol kinase expression in NIH Swiss mice.; Authors - Ni Zeng
human	WB	Life Sci Alliance; Serine palmitoyltransferase assembles at ER-mitochondria contact sites.; Authors - Mari J Aaltonen
human,mouse	IHC	J Adv Res; O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation.; Authors - Hongshuo Zhang
human,mouse	WB,IHC	Mol Metab; The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326; Authors - Brian E Ford
mouse	IF,RIP	Arterioscler Thromb Vasc Biol; Super Enhancer-Associated Circular RNA-CircKrt4 Regulates Hypoxic Pulmonary Artery Endothelial Cell Dysfunction in Mice; Authors - Cui Ma