Anticorps Monoclonal anti-Alpha Galactosidase A

• Phare
• Validé par KD/KO

Alpha Galactosidase A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2B2C5

N° de cat : 66121-1-Ig

Synonymes

GLA, 2B2C5, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Alpha-galactosidase A

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Galerie de données de validation

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  WB analysis of HEK-293 using 66121-1-Ig

  WB result of GLA antibody (66121-1-Ig, 1:2000) with si-Control and si-GLA transfected HEK293 cells..

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  WB analysis using 66121-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HeLa using 66121-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66121-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66121-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 66121-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 66121-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66121-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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  IF Staining of HepG2 using 66121-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha Galactosidase A antibody (66121-1-Ig, Clone: 2B2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules HepG2, cellules LNCaP, tissu rénal humain
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:10000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66121-1-Ig cible Alpha Galactosidase A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha Galactosidase A Protéine recombinante Ag7505
• Nom complet: galactosidase, alpha
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC002689
• Symbole du gène: GLA
• Identification du gène (NCBI): 2717
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLA, also named as Melibiase and Alpha-galactosidase A, belongs to the glycosyl hydrolase 27 family. It hydrolyzes terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.

Publications

Species	Application	Title
	WB	bioRxiv; Antigen-display exosomes provide adjuvant-free protection against SARS-CoV-2 disease at nanogram levels of spike protein; Authors - Chenxu Guo
human	WB	Nat Commun; Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis; Authors - Sebastian Boland

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