Anticorps Recombinant de lapin anti-GLAST/EAAT1

GLAST/EAAT1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241907H3

N° de cat : 84497-4-RR

Synonymes

GLAST, EAAT1, Sodium-dependent glutamate/aspartate transporter 1, SLC1A3, GLAST-1

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Galerie de données de validation

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  IF Staining of Neuro-2a using 84497-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using GLAST antibody (84497-4-RR, Clone: 241907H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U-937 using 84497-4-RR

  1x10^6 U-937 cells were intracellularly stained with 0.25 ug GLAST Recombinant antibody (84497-4-RR, Clone:241907H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HEK-293 using 84497-4-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GLAST Recombinant antibody (84497-4-RR, Clone:241907H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules Neuro-2a,
• Résultats positifs en FC (Intra): cellules HEK-293, cellules U-937

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84497-4-RR cible GLAST/EAAT1 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GLAST/EAAT1 Protéine recombinante Ag16962
• Nom complet: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• Masse moléculaire calculée: 542 aa, 60 kDa
• Numéro d’acquisition GenBank: BC037310
• Symbole du gène: GLAST
• Identification du gène (NCBI): 6507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)

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