Anticorps Monoclonal anti-GLI1

• Phare
• Validé par KD/KO

GLI1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(89)

CloneNo.

1D2B2

Avis (3)

N° de cat : 66905-1-Ig

Synonymes

1D2B2, GLI, GLI family zinc finger 1, Glioma associated oncogene, Glioma-associated oncogene

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Galerie de données de validation

• 

  WB analysis using 66905-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66905-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66905-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66905-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66905-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66905-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66905-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules 4T1, cellules BxPC-3, cellules C6, cellules HEK-293, cellules MCF-7, cellules MDA-MB-231, cellules NCI-H1299, cellules PC-3, cellules U-251, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 82 publications below
• IHC: See 17 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66905-1-Ig cible GLI1 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GLI1 Protéine recombinante Ag27832
• Nom complet: GLI family zinc finger 1
• Masse moléculaire calculée: 1106 aa, 118 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC013000
• Symbole du gène: GLI1
• Identification du gène (NCBI): 2735
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLI1, also known as Glioma-associated oncogene, is a 1106 amino acid protein, which belongs to the GLI C2H2-type zinc-finger protein family. GLI1 is detected in testis and Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. GLI1 is tethered in the cytoplasm by binding to SUFU (PubMed:10806483). GLI1 is activated and translocated to the nucleus promoted by interaction with STK36 (PubMed:10806483). Phosphorylation of GLI1 by ULK3 may promote nuclear localization (PubMed:19878745). GLI1 as a transcriptional activator may regulate the transcription of specific genes during normal development (PubMed:19706761). GLI1 plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract and Mediates SHH signaling (PubMed:19706761). GLI1 plays a role in cell proliferation and differentiation via its role in SHH signaling.

Publications

Species	Application	Title
mouse,human	WB	Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
human	WB,IHC	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
mouse	WB	J Nanobiotechnology; NaHS@Cy5@MS@SP nanoparticles improve rheumatoid arthritis by inactivating the Hedgehog signaling pathway through sustained and targeted release of H2S into the synovium; Authors - Xue-Xue Zhu
mouse	WB	JCI Insight; Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.; Authors - Hairong Shi
mouse	WB,IF,CoIP	Cancer Immunol Res; Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.; Authors - Qijing Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bárbara (Verified Customer) (07-31-2025)

We tested the GLI1 antibody (Proteintech) for Western blot in human PBMC lysates at 1:1000 dilution. The expected ~140 kDa band low, but with one band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PBMCs

FH

Boyan (Verified Customer) (07-23-2019)

In my experiment, I used the untreated and Shh ligand-treated cell lysates, in which the protein level of Gli1 should be increased after Shh treatment.With these two samples, I could detect a significant induction of the Gli1 protein using another Gli1 antibody. But, I could not see this induction with this antibody (66905-1-lg). A tricky thing is that, comparing with the real Gli1 band shown by the other antibody, this antibody (66905-1-lg) could recognise a protein at a similar molecular weight (but a little higher than that real Gli1). I think this is misleading, because although being shown on the website, that band is not the real band of Gli1.

• Applications: Western Blot,
• Cell Tissue Type: MEF

FH

Joshua (Verified Customer) (04-28-2019)

Cells paraformaldehyde fixed and stained for GLI1 (red). Counterstained with DRAQ7 (blue). Observed mixed nuclear/cytosolic staining as expected for GLI1.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse NIH3T3

GLI1 Antibody Immunofluorescence, validation (1:500 dilution) in Mouse NIH3T3 (Cat no:66905-1-Ig)

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