Anticorps Polyclonal de lapin anti-GLO1

• Phare
• Validé par KD/KO

GLO1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (1)

N° de cat : 15140-1-AP

Synonymes

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, glyoxalase I, Ketone aldehyde mutase, Lactoylglutathione lyase, Methylglyoxalase

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Galerie de données de validation

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  WB analysis using 15140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15140-1-AP (GLO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15140-1-AP

  IP result of anti-GLO1 (IP:15140-1-AP, 4ug; Detection:15140-1-AP 1:1000) with HepG2 cells lysate 1000ug.

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  IHC staining of human thyroid cancer using 15140-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15140-1-AP (GLO1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 15140-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15140-1-AP (GLO1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HeLa, cellules HepG2, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IF: See 1 publications below

Informations sur le produit

15140-1-AP cible GLO1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLO1 Protéine recombinante Ag7314
• Nom complet: glyoxalase I
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: BC001741
• Symbole du gène: GLO1
• Identification du gène (NCBI): 2739
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer. The molecular mass of the dimer was 46 kDa determined by gel permeation chromatography.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Paracrine Orchestration of Tumor Microenvironment Remodeling Induced by GLO1 Potentiates Lymph Node Metastasis in Breast Cancer; Authors - Jindong Xie
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
mouse	WB	Front Pharmacol; Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders mice; Authors - Jin-Bo Xie
mouse	WB,IF	Phytomedicine; Protective effects of Huang-Lian-Jie-Du Decoction on diabetic nephropathy through regulating AGEs/RAGE/Akt/Nrf2 pathway and metabolic profiling in db/db mice.; Authors - Dan Tang
human	WB	Oncotarget; Glyoxalase 1 gene is highly expressed in basal-like human breast cancers and contributes to survival of ALDH1-positive breast cancer stem cells.; Authors - Shoma Tamori ; KD Validated
mouse	WB	Life Sci Alliance; Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.; Authors - Shengya Tian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Billie (Verified Customer) (07-10-2021)

Strong band at around 25 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 with 10ug protein loaded
• Cell Tissue Type: Astrocytes

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