Anticorps Polyclonal de lapin anti-GLP1R

• Phare
• Validé par KD/KO

GLP1R Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(29)

Avis (2)

N° de cat : 26196-1-AP

Synonymes

GLP 1 R, GLP 1 receptor, GLP 1R, GLP-1 receptor, GLP-1R

---

Galerie de données de validation

• 

  WB analysis using 26196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26196-1-AP (GLP1R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 26196-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26196-1-AP (GLP1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, tissu pancréatique de rat
• Résultats positifs en IHC: tissu pulmonaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

26196-1-AP cible GLP1R dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLP1R Protéine recombinante Ag23529
• Nom complet: glucagon-like peptide 1 receptor
• Masse moléculaire calculée: 463 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC112126
• Symbole du gène: GLP1R
• Identification du gène (NCBI): 2740
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLP1R (glucagon-like peptide-1 receptor) is a G protein-coupled receptor belonging to the secretin receptor super-family, also known as class B (PMID:34911028). GLP1R is widely distributed in pancreatic islets, muscles, gastrointestinal tract, lung, liver, pancreas, and other tissues or organs (PMID:35989517). Moreover, GLP1R agonist has been reported to induce autophagy of EC (Endometrial carcinoma) cells, and high GLP1R expression may be associated with good prognosis of EC patients, suggesting that GLP1R is likely to participate in the progression of EC (PMID:29907137). Natural GLP-1R is known to be N-glycosylated at positions 63, 82 and 115kDa(PMID: 36769142). Moreover, regarding the molecular weight of GLP1R, research shows that the bands at ~50 kDa and ~100 kDa belong to the monomer and dimer states of GLP1R (PMID: 28609478).

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Geniposide Improves Diabetic Nephropathy by Enhancing ULK1-Mediated Autophagy and Reducing Oxidative Stress through AMPK Activation.; Authors - Theodomir Dusabimana
rat	WB	Life Sci; Human gestational diabetes mellitus-derived exosomes impair glucose homeostasis in pregnant mice and stimulate functional maturation of offspring-islets; Authors - Linhai Zou
mouse	WB	Biomed Pharmacother; Liraglutide, a glucagon-like peptide-1 receptor agonist, suppresses osteoclastogenesis through the inhibition of NF-κB and MAPK pathways via GLP-1R.; Authors - Ziyi Li ; KD Validated
	WB	Front Pharmacol; Novel Small Molecule Glucagon-Like Peptide-1 Receptor Agonist S6 Stimulates Insulin Secretion From Rat Islets.; Authors - Xiaohua Yang
rat	WB	Front Pharmacol; A Potential Mechanism Underlying the Therapeutic Effects of Progesterone and Allopregnanolone on Ketamine-Induced Cognitive Deficits.; Authors - Ting Cao
rat	WB	Front Endocrinol (Lausanne); Clozapine Induced Disturbances in Hepatic Glucose Metabolism: The Potential Role of PGRMC1 Signaling.; Authors - Ting Cao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kenzo (Verified Customer) (08-16-2023)

This KO validated GLP-1R antibody generated specific robust signals in mouse pancreas tissue when used for immunofluorescence

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

{{ptg:RelatedPrimaryAntibodies}}