Anticorps Polyclonal de lapin anti-GLRA1

GLRA1 Polyclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17951-1-AP

Synonymes

GLRA1, glycine receptor, alpha 1, STHE

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Galerie de données de validation

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  WB analysis of mouse brain using 17951-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17951-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 17951-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17951-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17951-1-AP (GLRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of SH-SY5Y using 17951-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GLRA1 antibody (17951-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y, tissu de cervelet de souris
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17951-1-AP cible GLRA1 dans les applications de WB, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLRA1 Protéine recombinante Ag12431
• Nom complet: glycine receptor, alpha 1
• Masse moléculaire calculée: 449 aa, 52 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC074980
• Symbole du gène: GLRA1
• Identification du gène (NCBI): 2741
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Publications

Species	Application	Title
mouse	CoIP	PLoS Biol; mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.; Authors - Zi-Yang Zhang
mouse	WB,IF	J Biol Chem; Clinical, genetic, and functional characterization of the glycine receptor β-subunit A455P variant in a family affected by hyperekplexia syndrome.; Authors - Ghada I Aboheimed
mouse	WB	Psychopharmacology (Berl); Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression.; Authors - Niannian Huang
mouse	WB	Eur J Pharmacol; Functional expression of glycine receptors in DRG neurons of mice.; Authors - Lin Yao
human	WB	Neuropharmacology; Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.; Authors - Zi-Yang Zhang