Anticorps Polyclonal de lapin anti-GLRA3

GLRA3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 13145-1-AP

Synonymes

GLRA3, glycine receptor, alpha 3

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Galerie de données de validation

• 

  WB analysis of MCF-7 using 13145-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13145-1-AP (GLRA3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

13145-1-AP cible GLRA3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLRA3 Protéine recombinante Ag3702
• Nom complet: glycine receptor, alpha 3
• Masse moléculaire calculée: 464 aa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC036086
• Symbole du gène: GLRA3
• Identification du gène (NCBI): 8001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Publications

Species	Application	Title
mouse	WB	PLoS Biol; mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.; Authors - Zi-Yang Zhang
human	WB	Neuropharmacology; Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.; Authors - Zi-Yang Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Braulio (Verified Customer) (02-26-2020)

Western blot in lumbar spinal cord samples, shows a mild band for alpha 3

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Spinal cord

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