Anticorps Polyclonal de lapin anti-GLRX

• Phare
• Validé par KD/KO

GLRX Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 15804-1-AP

Synonymes

Glutaredoxin 1, Glrx1, Glutaredoxin-1, Thioltransferase-1, TTase-1

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Galerie de données de validation

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  WB analysis using 15804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15804-1-AP (GLRX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15804-1-AP (GLRX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15804-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15804-1-AP (GLRX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 15804-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GLRX antibody (15804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Jurkat, lait humain, tissu placentaire humain, tissu pulmonaire de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 3 publications below

Informations sur le produit

15804-1-AP cible GLRX dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLRX Protéine recombinante Ag8535
• Nom complet: glutaredoxin (thioltransferase)
• Masse moléculaire calculée: 106 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC005304
• Symbole du gène: GLRX
• Identification du gène (NCBI): 2745
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	Elife; Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control; Authors - Meiling Wu
human	WB	Biochem Pharmacol; Ferroptosis induced by DCPS depletion diminishes hepatic metastasis in uveal melanoma; Authors - Bei Jin
human	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong ; KD Validated
human	WB	Sci Rep; Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.; Authors - Pengying Li
mouse	WB	J Bioenerg Biomembr; Glutaredoxin 1 protects neurons from oxygen-glucose deprivation/reoxygenation (OGD/R)-induced apoptosis and oxidative stress via the modulation of GSK-3β/Nrf2 signaling; Authors - Zhengguo Qiu ; KD Validated
human	WB	Mol Vis; Protective effect of Glutaredoxin 1 against oxidative stress in lens epithelial cells of age-related nuclear cataracts.; Authors - Qi Fan ; KD Validated

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