Anticorps Polyclonal de lapin anti-GLRX2

• Phare
• Validé par KD/KO

GLRX2 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13381-1-AP

Synonymes

GLRX2, Glutaredoxin 2, Glutaredoxin 2, mitochondrial, GRX2

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Galerie de données de validation

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  WB analysis using 13381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13381-1-AP (GLRX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 13381-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GLRX2 antibody (13381-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: HGC-27 cells, cellules MCF-7
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below

Informations sur le produit

13381-1-AP cible GLRX2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLRX2 Protéine recombinante Ag4218
• Nom complet: glutaredoxin 2
• Masse moléculaire calculée: 164 aa, 18 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC028113
• Symbole du gène: GLRX2
• Identification du gène (NCBI): 51022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLRX2(Glutaredoxin-2) is also named as GRX2 and belongs to the glutaredoxin family. It facilitates the maintenance of cellular redox homeostasis upon treatment with apoptotic agents, thereby preventing cardiolipin oxidation and cytochrome C release. The GLRX2, which exists in a dynamic equilibrium of enzymatically active monomers and quiescent dimers,is a 16-kDa protein directed either to the nucleus or to the mitochondria through differential splicing of the first exon(PMID: 17121859). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Free Radic Biol Med; Compartmentally scavenging hepatic oxidants through AMPK/SIRT3-PGC1α axis improves mitochondrial biogenesis and glucose catabolism.; Authors - Meiling Wu
human	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong ; KD Validated
mouse	WB	Int Immunopharmacol; Glutaredoxin 2 protects cardiomyocytes from hypoxia/reoxygenation-induced injury by suppressing apoptosis, oxidative stress, and inflammation via enhancing Nrf2 signaling.; Authors - Chengliang Li
mouse	WB	Brain Res; Upregulation of Glutaredoxin 2 alleviates oxygen-glucose deprivation/reoxygenation-induced apoptosis and ROS production in neurons by enhancing Nrf2 signaling via modulation of GSK-3β.; Authors - Jian Wen ; KD Validated
mouse	WB	J Physiol Sci; Redox proteins are constitutively secreted by skeletal muscle.; Authors - Yasuko Manabe