Anticorps Polyclonal de lapin anti-KGA-Specific

• Phare
• Validé par KD/KO

KGA-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 20170-1-AP

Synonymes

AAD20, GLS, GLS1, GLSK, glutaminase, K glutaminase, KGA, KGA-specific, KIAA0838, L glutamine amidohydrolase

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Galerie de données de validation

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  WB analysis of HEK-293 using 20170-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis using 20170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20170-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20170-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20170-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20170-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20170-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 20170-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 20170-1-AP

  IP Result of anti-KGA-Specific (IP:20170-1-AP, 3ug; Detection:20170-1-AP 1:800) with HEK-293 cells lysate 3200ug.

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  IHC staining of human kidney using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse kidney using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 20170-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 20170-1-AP (KGA-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 20170-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KGA-Specific antibody (20170-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

20170-1-AP cible KGA-Specific dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KGA-Specific Protéine recombinante Ag14002
• Nom complet: glutaminase
• Masse moléculaire calculée: 669 aa, 73 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC038507
• Symbole du gène: GLS
• Identification du gène (NCBI): 2744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.

Publications

Species	Application	Title
mouse	WB	Nat Med; Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia.; Authors - Daniel Herranz
mouse	WB	Cell Stem Cell; CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.; Authors - Gianluca L Russo
human	WB	Blood; Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.; Authors - Nathalie Jacque
rat	CoIP, WB	Int J Mol Sci; Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.; Authors - Małgorzata Beręsewicz-Haller
human	WB,IHC	EBioMedicine; Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.; Authors - Binghua Li
human	WB	J Genet Genomics; A facile and sensitive method of quantifying glutaminase binding to its inhibitor CB-839 in tissues.; Authors - Yicheng Chen