Anticorps Polyclonal de lapin anti-KGA/GAM/GAC

• Phare
• Validé par KD/KO

KGA/GAM/GAC Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 23549-1-AP

Synonymes

AAD20, GAC, GAM, GLS, GLS1, glutaminase, K glutaminase, KGA, KGA/GAM/GAC, KIAA0838, L glutamine amidohydrolase

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 23549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 23549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 23549-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23549-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 23549-1-AP

  IP Result of anti-KGA/GAM/GAC (IP:23549-1-AP, 4ug; Detection:23549-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human kidney using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IHC staining of human brain using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IHC staining of human brain using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IF Staining of HEK-293 using 23549-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 23549-1-AP (KGA/GAM/GAC antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 23549-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HeLa using 23549-1-AP

  Immunofluorescent analysis of HeLa cells using 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:25-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

23549-1-AP cible KGA/GAM/GAC dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KGA/GAM/GAC Protéine recombinante Ag20354
• Nom complet: glutaminase
• Masse moléculaire calculée: 669 aa, 73 kDa
• Poids moléculaire observé: 58 kDa, 65 kDa, 83 kDa
• Numéro d’acquisition GenBank: BC038507
• Symbole du gène: GLS
• Identification du gène (NCBI): 2744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa(PMID:12408749). Ping Gao et al. (2009) determined that mitochondrial glutaminase expression (GLS, molecular mass of ~58 kDa) is increased ~10-fold in response to Myc. It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). GLS has 3 isoforms produced by alternative splicing and this antibody can recognize all the 3 isoforms(KGA,GAM,GAC) of GLS.

Publications

Species	Application	Title
human	WB,IF	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KO Validated
human	WB	Redox Biol; Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels.; Authors - M Ryan Smith