Anticorps Polyclonal de lapin anti-Glutamine Synthetase

• Phare
• Validé par KD/KO

Glutamine Synthetase Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(39)

Avis (4)

N° de cat : 11037-2-AP

Synonymes

GLUL, EC:2.3.1.225, EC:6.3.1.2, GLNS, Glutamate--ammonia ligase

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Galerie de données de validation

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  WB analysis of Jurkat using 11037-2-AP

  WB result of Glutamine synthetase antibody (11037-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells.

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  WB analysis using 11037-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11037-2-AP

  IP result of anti-Glutamine Synthetase (IP:11037-2-AP, 4ug; Detection:11037-2-AP 1:5000) with mouse liver tissue lysate 2400 ug.

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  IHC staining of mouse liver using 11037-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 11037-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 11037-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 11037-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human brain using 11037-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human brain tissue using 11037-2-AP (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 11037-2-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug Glutamine Synthetase Polyclonal antibody (11037-2-AP)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris, tissu cérébral humain, tissu oculaire de souris
• Résultats positifs en FC (Intra): cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 30 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

11037-2-AP cible Glutamine Synthetase dans les applications de WB, IHC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glutamine Synthetase Protéine recombinante Ag1510
• Nom complet: glutamate-ammonia ligase (glutamine synthetase)
• Masse moléculaire calculée: 374 aa, 42 kDa
• Poids moléculaire observé: 40-42 kDa
• Numéro d’acquisition GenBank: BC011700
• Symbole du gène: Glutamine Synthetase
• Identification du gène (NCBI): 2752
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).

Publications

Species	Application	Title
mouse	IF	Nat Methods; Spatially resolved isotope tracing reveals tissue metabolic activity.; Authors - Lin Wang
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou ; KD Validated
mouse,human	WB,IF	Protein Cell; Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation; Authors - Kai-Hui Zhang
human	WB	Sci Transl Med; An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs.; Authors - Wei-Jian Li
mouse	WB	Cell Res; Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.; Authors - Wei-Feng Cai ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marisa (Verified Customer) (07-29-2025)

I've used this antibody for immunohistochemistry in brain slices followed by confocal microscopy, and it performed consistently well. Clear signal, minimal background, and good lot-to-lot consistency. The datasheet and customer support were also helpful. Highly recommend for confocal applications.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1-250
• Cell Tissue Type: Brain

FH

Andrea (Verified Customer) (09-06-2023)

antibody works perfect

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse retina

FH

Melanie (Verified Customer) (02-02-2023)

Good antibody, which works well in HEK293T cells

• Applications: Western Blot
• Cell Tissue Type: HEK293T cells

FH

Ryan (Verified Customer) (02-27-2019)

Tris-HCL antigen retrieval ph=9.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina (Muller glia) at 3dpf

Glutamine Synthetase Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Muller glia) at 3dpf (Cat no:11037-2-AP)

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