Anticorps Polyclonal de lapin anti-GLUT5

• Phare
• Validé par KD/KO

GLUT5 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 27571-1-AP

Synonymes

Fructose transporter, GLUT 5, GLUT5, SLC2A5

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Galerie de données de validation

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  WB analysis of A549 using 27571-1-AP

  WB result of GLUT5 antibody (27571-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLUT5 transfected A549 cells.

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  WB analysis of Caco-2 using 27571-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 27571-1-AP (GLUT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27571-1-AP (GLUT5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 27571-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27571-1-AP (GLUT5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27571-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27571-1-AP (GLUT5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27571-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27571-1-AP (GLUT5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27571-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27571-1-AP (GLUT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules A549, tissu cérébral de souris, tissu rénal de souris, tissu testiculaire de rat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 3 publications below

Informations sur le produit

27571-1-AP cible GLUT5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLUT5 Protéine recombinante Ag25682
• Nom complet: solute carrier family 2 (facilitated glucose/fructose transporter), member 5
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 48-70 kDa
• Numéro d’acquisition GenBank: BC001820
• Symbole du gène: SLC2A5
• Identification du gène (NCBI): 6518
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLUT5, also known as SLC2A5, is a fructose transporter expressed on the apical border of enterocytes in the small intestine. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen (PMID: 12750891). GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue (adipocytes), and brain (PMID: 9781312, 18398011).

Publications

Species	Application	Title
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	WB	Nutrients; Acute Low-Intensity Treadmill Running Upregulates the Expression of Intestinal Glucose Transporters via GLP-2 in Mice.; Authors - Kai Aoki
mouse	WB	iScience; Celastrol inhibits intestinal lipid absorption by reprofiling the gut microbiota to attenuate high-fat diet-induced obesity.; Authors - Hu Hua
human	WB	Dev Neurobiol; Prestin-prestin and prestin-GLUT5 interactions in HEK293T cells.; Authors - Xudong Wu
mouse	WB	Cell Metab; Dietary fructose-mediated adipocyte metabolism drives antitumor CD8+ T cell responses; Authors - Yuerong Zhang
mouse,human	WB	Anim Cells Syst (Seoul); Role of Carbohydrate response element-binding protein in mediating dexamethasone-induced glucose transporter 5 expression in Caco-2BBE cells and during the developmental phase in mice; Authors - Soonjae Hwang