Anticorps Recombinant de lapin anti-GM-CSF

GM-CSF Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

243117B1

N° de cat : 98463-3-RR

Synonymes

Colony-stimulating factor, CSF, Csf2, Csfgm, Granulocyte-macrophage colony-stimulating factor

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using 98463-3-RR

  1x10^6 HEK-293T cells or GM-CSF transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat GM-CSF Rabbit RecAb (98463-3-RR, Clone: 243117B1) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293T using 98463-3-RR

  1x10^6 HEK-293T cells or GM-CSF transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat GM-CSF/CSF2 Rabbit RecAb (98463-3-RR, Clone: 243117B1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98463-3-RR cible GM-CSF dans les applications de FC (Intra) et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GM-CSF Protéine recombinante Eg3202
• Nom complet: colony stimulating factor 2 (granulocyte-macrophage)
• Masse moléculaire calculée: 17 kDa
• Numéro d’acquisition GenBank: NM_053852.1
• Symbole du gène: Csf2
• Identification du gène (NCBI): 116630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Gm-csf, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. Gm-csf was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. Gm-csf is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. Gm-csf has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.

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