Anticorps Polyclonal de lapin anti-GM2A

• Phare
• Validé par KD/KO

GM2A Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 10864-2-AP

Synonymes

Sphingolipid activator protein 3, SAP-3, SAP3, GM2-AP, GM2AP

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Galerie de données de validation

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  WB analysis of rat kidney using 10864-2-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10864-2-AP (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10864-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10864-2-AP (GM2A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10864-2-AP

  IP result of anti-GM2A (IP:10864-2-AP, 3ug; Detection:10864-2-AP 1:600) with HEK-293 cells lysate 2800ug.

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  IHC staining of human placenta using 10864-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10864-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 10864-2-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (10864-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

10864-2-AP cible GM2A dans les applications de WB, IHC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GM2A Protéine recombinante Ag1295
• Nom complet: GM2 ganglioside activator
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC009273
• Symbole du gène: GM2A
• Identification du gène (NCBI): 2760
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

Publications

Species	Application	Title
rat	WB	Basic Res Cardiol; Expression and regulation of type 2A protein phosphatases and alpha4 signalling in cardiac health and hypertrophy.; Authors - Olga Eleftheriadou
human	WB	Cell Struct Funct; Glycosphingolipid GM2 Induces Invasiveness in Irradiation-tolerant Lung Cancer Cells.; Authors - Seiichiro Ishihara ; KD Validated
human	WB	Mol Neurodegener; Elevated ganglioside GM2 activator (GM2A) in human brain tissue reduces neurite integrity and spontaneous neuronal activity; Authors - Yi-Chen Hsieh
human	WB	Nat Commun; Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis; Authors - Sebastian Boland

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