Anticorps Polyclonal de lapin anti-GMDS

GMDS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15442-1-AP

Synonymes

SDR3E1, GMD, GDP-mannose 4,6 dehydratase, GDP-D-mannose dehydratase, EC:4.2.1.47

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Galerie de données de validation

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  WB analysis using 15442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 15442-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15442-1-AP (GMDS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15442-1-AP (GMDS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15442-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GMDS antibody (15442-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, cellules HeLa, tissu pancréatique de rat, tissu splénique de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15442-1-AP cible GMDS dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GMDS Protéine recombinante Ag7319
• Nom complet: GDP-mannose 4,6-dehydratase
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 40-42 kDa
• Numéro d’acquisition GenBank: BC000117
• Symbole du gène: GMDS
• Identification du gène (NCBI): 2762
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GDP-D-mannose-4,6-dehydratase (GMDS) is the key enzyme in the 'de novo' pathway of GDP-L-fucose biosynthesis(PMID:10462046). The enzyme is also named as GMD and belongs to the GDP-mannose 4,6-dehydratase family. The gene encodes a 42 kDa protein that catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor.

Publications

Species	Application	Title
human	WB,IP	Mol Cell Biol; GDP-mannose-4,6-dehydratase is a cytosolic partner of tankyrase 1 that inhibits its poly(ADP-ribose) polymerase activity.; Authors - Bisht Kamlesh K KK
human	WB	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei.; Authors - Lilli Saarinen
human	WB	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei; Authors - Lilli Saarinen
human	WB	Cell Chem Biol; High-Sensitivity and Low-Toxicity Fucose Probe for Glycan Imaging and Biomarker Discovery.; Authors - Yasuhiko Kizuka
human	WB,IF	Stem Cell Reports; Low fucosylation defines the glycocalyx of progenitor cells and melanocytes in the human limbal stem cell niche; Authors - Ashley M Woodward

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