Anticorps Polyclonal de lapin anti-GMFG

GMFG Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 13625-1-AP

Synonymes

Glia maturation factor gamma, glia maturation factor, gamma, GMF GAMMA, GMFG

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Galerie de données de validation

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  WB analysis of rat brain using 13625-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13625-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 13625-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13625-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 13625-1-AP

  IP Result of anti-GMFG (IP:13625-1-AP, 4ug; Detection:13625-1-AP 1:500) with mouse spleen tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13625-1-AP (GMFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13625-1-AP (GMFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13625-1-AP (GMFG Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13625-1-AP (GMFG Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 13625-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cardiaque humain, tissu placentaire de souris, tissu placentaire humain
• Résultats positifs en IP: tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cardiaque humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

13625-1-AP cible GMFG dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GMFG Protéine recombinante Ag4538
• Nom complet: glia maturation factor, gamma
• Masse moléculaire calculée: 141 aa, 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC032819
• Symbole du gène: GMFG
• Identification du gène (NCBI): 9535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GMFG, is a highly conserved brain-specific protein that belongs to the GMF subfamily of the larger Actin-binding protein ADF family. GMFG is preferentially expressed in blood vessel cells and immune cells and its ectopic expression enhances cellular functions such as tube-formation and migration in vitro. GMFG mediates neutrophil and T-lymphocyte migration via regulation of actin cytoskeletal reorganization. GMFG also acts as an intracellular regulator of stress-activated signal transduction by demonstrating activation of p38 MAP kinase and transcription factor NF-kB in astrocytes.

Publications

Species	Application	Title
mouse	IHC	Development; Coronary veins determine the pattern of sympathetic innervation in the developing heart.; Authors - Nam Joseph J
human	WB,IF	Front Cell Dev Biol; The Actin-Disassembly Protein Glia Maturation Factor γ Enhances Actin Remodeling and B Cell Antigen Receptor Signaling at the Immune Synapse.; Authors - Nikola Deretic ; KD Validated
human	IHC	Front Oncol; GMFG Has Potential to Be a Novel Prognostic Marker and Related to Immune Infiltrates in Breast Cancer.; Authors - Yan Yang
		Oncol Rep; Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro.; Authors - Huili Wang
human	WB,IHC	Genes (Basel); High Level of GMFG Correlated to Poor Clinical Outcome and Promoted Cell Migration and Invasion through EMT Pathway in Triple-Negative Breast Cancer; Authors - Yonglin Zhao
human	WB	Med Oncol; RNF144A exerts tumor suppressor function in breast cancer through targeting YY1 for proteasomal degradation to downregulate GMFG expression.; Authors - Yin-Ling Zhang