Anticorps Polyclonal de lapin anti-GMPS

GMPS Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 16376-1-AP

Synonymes

Glutamine amidotransferase, GMP synthetase, GMPS

---

Galerie de données de validation

• 

  WB analysis of HeLa using 16376-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16376-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 16376-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16376-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16376-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16376-1-AP

  IP result of anti-GMPS (IP:16376-1-AP, 4ug; Detection:16376-1-AP 1:800) with HeLa cells lysate 1080ug.

• 

  IHC staining of human liver cancer using 16376-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16376-1-AP (GMPS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 16376-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16376-1-AP (GMPS antibody) at dilution of 1:100 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HL-60, cellules Jurkat, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 3 publications below

Informations sur le produit

16376-1-AP cible GMPS dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GMPS Protéine recombinante Ag9350
• Nom complet: guanine monphosphate synthetase
• Masse moléculaire calculée: 693 aa, 77 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC012178
• Symbole du gène: GMPS
• Identification du gène (NCBI): 8833
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
human	WB, IHC	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv
human	WB	Aging (Albany NY); The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.; Authors - Jing Wang
human	WB,IHC	J Biomed Sci; TRIM21-SERPINB5 aids GMPS repression to protect nasopharyngeal carcinoma cells from radiation-induced apoptosis.; Authors - Panpan Zhang
mouse	WB	Cell Rep; Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.; Authors - Nicole M Sayles
human,mouse	WB,IHC	J Proteome Res; Comprehensive Urinary Proteome Profiling Analysis Identifies Diagnosis and Relapse Surveillance Biomarkers for Bladder Cancer; Authors - Qi Chang

{{ptg:RelatedPrimaryAntibodies}}