Anticorps Polyclonal de lapin anti-GNPDA1

• Phare
• Validé par KD/KO

GNPDA1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 12312-1-AP

Synonymes

GlcN6P deaminase 1, GNPDA, GNPDA 1, GNPDA1, GNPI, GPI, HLN, KIAA0060, Oscillin

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Galerie de données de validation

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  WB analysis of K-562 using 12312-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12312-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 12312-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12312-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12312-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12312-1-AP (GNPDA1 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, tissu cérébral humain, tissu rénal de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

12312-1-AP cible GNPDA1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNPDA1 Protéine recombinante Ag2961
• Nom complet: glucosamine-6-phosphate deaminase 1
• Masse moléculaire calculée: 289 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC012853
• Symbole du gène: GNPDA1
• Identification du gène (NCBI): 10007
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNPDA1(glucosamine-6-phosphate isomerase 1) is also named as GNPI, HLN, KIAA0060 and belongs to the glucosamine/galactosamine-6-phosphate isomerase family. It catalyzes the reversible conversion of D-glucosamine-6-phosphate (GlcN6P) into D-fructose-6-phosphate (Fru6P) and ammonium(PMID:12965206). It may be playing an essential role for egg activation and early embryo development. GNPI is expressed as a 33-kD cytosolic protein in various cell lines(PMID: 9714720).

Publications

Species	Application	Title
human	IHC	Front Cell Dev Biol; Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.; Authors - Yajuan Zhao
human	WB	Cancer Manag Res; Glucosamine-6-Phosphate Isomerase 1 Promotes Tumor Progression and Indicates Poor Prognosis in Hepatocellular Carcinoma.; Authors - Dezhi Li ; KD Validated
human	WB	Glycobiology; Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.; Authors - Sanna Oikari ; KD Validated

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