Anticorps Polyclonal de lapin anti-GNPDA2

• Phare
• Validé par KD/KO

GNPDA2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 17105-1-AP

Synonymes

GlcN6P deaminase 2, GNP2, GNPDA 2, GNPDA2, SB52

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Galerie de données de validation

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  WB analysis of rat kidney using 17105-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17105-1-AP (GNPDA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17105-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17105-1-AP (GNPDA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse kidney using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal de souris, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

17105-1-AP cible GNPDA2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNPDA2 Protéine recombinante Ag10780
• Nom complet: glucosamine-6-phosphate deaminase 2
• Masse moléculaire calculée: 275 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC015532
• Symbole du gène: GNPDA2
• Identification du gène (NCBI): 132789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNPDA2(Glucosamine-6-phosphate deaminase 2) is also named as GNP2 and belongs to the glucosamine/galactosamine-6-phosphate isomerase family. It catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. Human GNPDA1 and GNPDA2 shared the same fold and were predicted to form hexameric particles(PMID:12965206). This protein has 5 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 29 kDa, 23 kDa and 27 kDa.

Publications

Species	Application	Title
human	WB	Glycobiology; Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.; Authors - Sanna Oikari ; KD Validated
mouse	WB	Int J Endocrinol; GNPDA2 Gene Affects Adipogenesis and Alters the Transcriptome Profile of Human Adipose-Derived Mesenchymal Stem Cells.; Authors - Lijun Wu

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