Anticorps Polyclonal de lapin anti-GOLGA3

GOLGA3 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (1)

N° de cat : 21193-1-AP

Synonymes

GCP170, Golgi complex-associated protein of 170 kDa, Golgin 160, Golgin-160, MEA 2

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Galerie de données de validation

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  WB analysis using 21193-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21193-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21193-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 21193-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOLGA3 antibody (21193-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules L02, cellules PC-3, tissu testiculaire humain
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IF: See 2 publications below

Informations sur le produit

21193-1-AP cible GOLGA3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GOLGA3 Protéine recombinante Ag15531
• Nom complet: golgi autoantigen, golgin subfamily a, 3
• Masse moléculaire calculée: 1498 aa, 167 kDa
• Poids moléculaire observé: 167 kDa
• Numéro d’acquisition GenBank: BC142658
• Symbole du gène: GOLGA3
• Identification du gène (NCBI): 2802
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.; Authors - Gunjan Joshi
human	WB	PLoS Biol; A  conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.; Authors - Weichao Zhang
		J Cell Sci; SIRT2 deacetylates GRASP55 to facilitate post-mitotic Golgi assembly.; Authors - Xiaoyan Zhang
human	WB	J Biol Chem; GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.; Authors - Erpan Ahat
human	WB	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
mouse,human	WB,IF	PeerJ; Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis; Authors - Changtong Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-02-2023)

Very good for WB.

• Applications: Western Blot

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