Anticorps Polyclonal de lapin anti-GP73/GOLPH2

• Phare
• Validé par KD/KO

GP73/GOLPH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 15126-1-AP

Synonymes

GOLM1, GOLPH2, GP73, GOLM 1, golgi protein 73kD

---

Galerie de données de validation

• 

  WB analysis using 15126-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15126-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15126-1-AP

  IP result of anti-GP73/GOLPH2 (IP:15126-1-AP, 4ug; Detection:15126-1-AP 1:1000) with HeLa cells lysate 2800ug.

• 

  IHC staining of human stomach using 15126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15126-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15126-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15126-1-AP (GP73/GOLPH2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton and nuclei were stained with CL594-phalloidin and DAPI, respectively.

• 

  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15126-1-AP (GP73/GOLPH2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staning with CoraLite555-Phalloidin.

---

Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HepG2, cellules HuH-7, cellules MDA-MB-231
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du foie humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 7 publications below

Informations sur le produit

15126-1-AP cible GP73/GOLPH2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GP73/GOLPH2 Protéine recombinante Ag7207
• Nom complet: golgi membrane protein 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC001740
• Symbole du gène: GP73
• Identification du gène (NCBI): 51280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.

Publications

Species	Application	Title
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
human	WB	PLoS Pathog; GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.; Authors - Xuewu Zhang ; KD Validated
human	WB	J Cell Sci; Rapid affinity purification of intracellular organelles using a twin strep tag.; Authors - Jian Xiong
human	IF	Chemistry; Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction; Authors - Sayan Kundu
mouse	WB,IF	Int J Med Sci; Causal Association of Golgi Protein 73 With Coronary Artery Disease: Evidence from Proteomics and Mendelian Randomization; Authors - Yi-Fen Lin
human	WB,IHC,IF	J Cancer; GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.; Authors - Jia-Zhou Ye ; KD Validated

{{ptg:RelatedPrimaryAntibodies}}