Anticorps Polyclonal de lapin anti-GOSR2/Membrin

• Phare
• Validé par KD/KO

GOSR2/Membrin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 12095-1-AP

Synonymes

Membrin, GS27, GOSR2, Golgi SNAP receptor complex member 2, 27 kDa Golgi SNARE protein

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Galerie de données de validation

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  WB analysis of Jurkat using 12095-1-AP

  WB result of GOSR2/Membrin antibody (12095-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells.

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  WB analysis using 12095-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12095-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12095-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 12095-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 12095-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12095-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOSR2/Membrin antibody (12095-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 12095-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12095-1-AP

  Immunofluorescent analysis of () fixed HepG2 cells using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules L02, tissu d'estomac de souris, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12095-1-AP cible GOSR2/Membrin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GOSR2/Membrin Protéine recombinante Ag2736
• Nom complet: golgi SNAP receptor complex member 2
• Masse moléculaire calculée: 212 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC009710
• Symbole du gène: Membrin
• Identification du gène (NCBI): 9570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models; Authors - Ying Xue Xie
	WB	Commun Biol; Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.; Authors - Xihua Yue

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