Anticorps Polyclonal de lapin anti-GOT2

• Phare
• Validé par KD/KO

GOT2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 14800-1-AP

Synonymes

FABPpm, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A

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Galerie de données de validation

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  WB analysis using 14800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14800-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14800-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14800-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14800-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14800-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 14800-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GOT2 antibody 14800-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HEK-293 using 14800-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug GOT2 antibody (14800-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HepG2, cerveau de rat, cerveau de souris, tissu cardiaque de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque de rat, tissu cérébral de rat, tissu cutané humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 28 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14800-1-AP cible GOT2 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GOT2 Protéine recombinante Ag6514
• Nom complet: glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC000525
• Symbole du gène: GOT2
• Identification du gène (NCBI): 2806
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

Publications

Species	Application	Title
mouse	WB	Cell Stem Cell; Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.; Authors - Le Qi ; KO Validated
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
human	WB,CoIP	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
mouse	WB	Cell Res; Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.; Authors - Wei-Feng Cai ; KD Validated
human	WB	Nat Commun; Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.; Authors - Yun-Qian Wang
human	WB,IHC	Nat Commun; Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.; Authors - Maren Feist

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