Anticorps Recombinant de lapin anti-GP73/GOLPH2

GP73/GOLPH2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1I12

N° de cat : 81893-1-RR

Synonymes

GOLM1, GOLPH2, GP73, 1I12, C9orf155

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Galerie de données de validation

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  WB analysis using 81893-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 81893-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovarian cancer using 81893-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 81893-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 81893-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 81893-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 81893-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 81893-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:450 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 81893-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 81893-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81893-1-RR against Human GP73/GOLPH2 were performed. The affinity constant is 0.828 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du foie humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:450-1:1800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81893-1-RR cible GP73/GOLPH2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GP73/GOLPH2 Protéine recombinante Ag7207
• Nom complet: golgi membrane protein 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC001740
• Symbole du gène: GP73
• Identification du gène (NCBI): 51280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.

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