Anticorps Polyclonal de lapin anti-GPBP1

• Phare
• Validé par KD/KO

GPBP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 21622-1-AP

Synonymes

GPBP, GPBP1, SSH6, Vascular wall linked protein, VASCULIN

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Galerie de données de validation

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  WB analysis of HepG2 using 21622-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21622-1-AP (GPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21622-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21622-1-AP (GPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 10x lens).

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  IHC staining of human small intestine using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 40x lens).

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  IHC staining of human small intestine using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine slide using 21622-1-AP (GPBP1 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 21622-1-AP (GPBP1 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

21622-1-AP cible GPBP1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPBP1 Protéine recombinante Ag14877
• Nom complet: GC-rich promoter binding protein 1
• Masse moléculaire calculée: 473 aa, 53 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC000267
• Symbole du gène: GPBP1
• Identification du gène (NCBI): 65056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IP	Exp Mol Med; Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells.; Authors - Liang-Liang Fan ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joseph (Verified Customer) (04-30-2025)

Protein amount used for detection: 15–40 μg total protein per well Specificity: Validated with GPBP1 KO cells; some non-specific bands observed Molecular weight: Detects two bands around 70 kDa Blocking: 5% BSA in PBS + 0.1% Tween 20 (5% milk caused high background) Primary antibody: 1:1500, 1 h at RT Wash: 3–4 × 5 min with PBS + 0.1% Tween 20

• Applications: Western Blot
• Primary Antibody Dilution: 1/1500
• Cell Tissue Type: HCT116 and HEK293

GPBP1 Antibody Western Blot validation (1/1500 dilution) in HCT116 and HEK293 (Cat no:21622-1-AP)

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