Anticorps Polyclonal de lapin anti-GPR107

• Phare
• Validé par KD/KO

GPR107 Polyclonal Antibody for FC, IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 25076-1-AP

Synonymes

G protein coupled receptor 107, GCDRP, GPR107, KIAA1624, LUSTR1, Protein GPR107, RP11 88G17

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 25076-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25076-1-AP (GPR107 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 25076-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25076-1-AP (GPR107 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 25076-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25076-1-AP (GPR107 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of SH-SY5Y using 25076-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GPR107 antibody (25076-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu de cervelet de souris
• Résultats positifs en IF: cellules HEK-293
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

25076-1-AP cible GPR107 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPR107 Protéine recombinante Ag18649
• Nom complet: G protein-coupled receptor 107
• Masse moléculaire calculée: 552 aa, 67 kDa
• Poids moléculaire observé: 60-67 kDa
• Numéro d’acquisition GenBank: BC110518
• Symbole du gène: GPR107
• Identification du gène (NCBI): 57720
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPR107 also named as KIAA1624 or LUSTR1 is 600 amino acid protein, which belongs to the LU7TM family. GPR107 localizes in membrane and is a multi-pass membrane protein. GPR107 is a promising candidate receptor for neuronostatin, and neuronostatin, interacting with GPR107, may play an important role in the central control of cardiovascular function.

Publications

Species	Application	Title
mouse	WB	Nat Chem Biol; A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes.; Authors - Yu-Shiuan Cheng
human	WB,IHC,IF	J Exp Clin Cancer Res; LncGPR107 drives the self-renewal of liver tumor initiating cells and liver tumorigenesis through GPR107-dependent manner.; Authors - Guanqun Huang
mouse	WB	Neurochem Res; Neuronostatin Promotion Soluble Aβ1-42 Oligomers: Induced Dysfunctional Brain Glucose Metabolism in Mice.; Authors - Shaobin Yang
mouse	WB	Behav Brain Res; Neuronostatin promotes soluble Aβ1-42 oligomers -induced spatial learning and memory impairments in mice.; Authors - Shaobin Yang ; KD Validated
human	WB	Nat Chem Biol; Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A; Authors - Xiaohui Zhao