Anticorps Polyclonal de lapin anti-GPR50

• Phare
• Validé par KD/KO

GPR50 Polyclonal Antibody for FC, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 19762-1-AP

Synonymes

G protein coupled receptor 50, GPR50, H9, Mel1c, Melatonin related receptor

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Galerie de données de validation

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  IHC staining of mouse brain using 19762-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19762-1-AP (GPR50 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 19762-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19762-1-AP (GPR50 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain tissue using 19762-1-AP

  IF result of anti-GPR50 (19762-1-AP, 1:50) with mouse brain tissue slide by Dr. Qian Li, University of Edinburgh.

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  IF Staining of SH-SY5Y cells using 19762-1-AP

  IF result of anti-GPR50 (19762-1-AP, 1:500) with SH-SY5Y cells transfected with GPR50 by Dr. Qian Li, University of Edinburgh.

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  IF Staining of mouse brain using 19762-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR50 antibody (19762-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 19762-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR50 antibody (19762-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 19762-1-AP

  1X10^6 K-562 cells were stained with 0.2ug GPR50 antibody (19762-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris, cellules SH-SY5Y
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

19762-1-AP cible GPR50 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: G protein-coupled receptor 50
• Masse moléculaire calculée: 67 kDa
• Numéro d’acquisition GenBank: NM_004224
• Symbole du gène: GPR50
• Identification du gène (NCBI): 9248
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPR50, also known as Melatonin-related receptor or H9, is a member of the G-protein coupled receptor 1 family. It is closely related to the G protein-coupled melatonin receptor family and has been demonstrated as the mammalian ortholog of Mel1c receptor (PMID: 8647286; 18400093). GPR50 does not bind melatonin. It is able to heterodimerize with both the MT1 (Mel1a) and MT2 (Mel1b) melatonin receptor subtypes. While GPR50 has no effect on MT2 function, GPR50 prevented MT1 from both binding melatonin and coupling to G proteins. GPR50 is involved in neuroendocrine function and plays a role in the regulation of energy metabolism (PMID: 8647286; 17957037).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
mouse	WB,IP	Transl Stroke Res; Melatonin Offers Dual-Phase Protection to Brain Vessel Endothelial Cells in Prolonged Cerebral Ischemia-Recanalization Through Ameliorating ER Stress and Resolving Refractory Stress Granule; Authors - Danli Lu
human	IF	Front Pharmacol; An Improved Genetically Encoded Fluorescent cAMP Indicator for Sensitive cAMP Imaging and Fast Drug Screening.; Authors - Wenfeng Liu ; KO Validated
human	WB	Am J Chin Med; Tetramethylpyrazine Attenuated Sevoflurane-Induced Neurotoxicity by Enhancing Autophagy through GPR50/CREB Pathway in SH-SY5Y Cells.; Authors - Lili Xu ; KD Validated
mouse	WB	FEBS Open Bio; Orphan receptor GPR50 attenuates inflammation and insulin signaling in 3T3-L1 preadipocytes; Authors - Zhenyu Yao ; KO Validated

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