Anticorps Polyclonal de lapin anti-GPRC5A,RAI3

• Phare
• Validé par KD/KO

GPRC5A,RAI3 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10309-1-AP

Synonymes

GPCR5A, GPRC5A, GPRC5A,RAI3, RAI3, RAIG 1, RAIG1

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Galerie de données de validation

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  WB analysis using 10309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10309-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of MCF-7 using 10309-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug GPRC5A,RAI3 antibody (10309-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HeLa, cellules MCF-7, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

10309-1-AP cible GPRC5A,RAI3 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Chèvre, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPRC5A,RAI3 Protéine recombinante Ag0311
• Nom complet: G protein-coupled receptor, family C, group 5, member A
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC003665
• Symbole du gène: GPRC5A
• Identification du gène (NCBI): 9052
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPRC5A, also named as GPCR5A, RAI3 and RAIG1, belongs to the G-protein coupled receptor 3 family. It is an orphan G-protein coupled receptor (GPCR) that has been associated with malignancy and may play a role in the proliferation of breast cancer cells. GPRC5A is a potential molecular target for treatment of breast cancers. It is suppressed in some human lung carcinoma cells. (PMID:15788639, 20563252, 19552806 )

Publications

Species	Application	Title
goat	IHC	Cell Prolif; Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysis; Authors - Peng Lin
human	WB	Sci Total Environ; GPRC5A reduction contributes to pollutant benzo[a]pyrene injury via aggravating murine fibrosis, leading to poor prognosis of IIP patients.; Authors - Ziling Huang ; KO Validated
human	WB	Oncol Lett; Clinical significance of reduced GPRC5A expression in surgically resected non-small cell lung cancer.; Authors - Er Jin
human	WB	Exp Lung Res; GPRC5a suppresses the proliferation of non-small cell lung cancer under wild type p53 background.; Authors - Jin Er
		Mol Med Rep; Lung cancer suppressor gene GPRC5A mediates p53 activity in non‑small cell lung cancer cells in vitro.; Authors - Er Jin
human	WB	Stem Cell Res Ther; TMT-based quantitative proteomic analysis of spheroid cells of endometrial cancer possessing cancer stem cell properties; Authors - Mingzhu Cao