Anticorps Polyclonal de lapin anti-GPRC5A,RAI3

• Phare
• Validé par KD/KO

GPRC5A,RAI3 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 10309-1-AP

Synonymes

GPRC5A, GPCR5A, G-protein coupled receptor family C group 5 member A, PEIG-1, Phorbol ester induced gene 1

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Galerie de données de validation

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  WB analysis using 10309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10309-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung squamous cell carcinoma using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 10309-1-AP (GPRC5A,RAI3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of HeLa using 10309-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug GPRC5A,RAI3 Polyclonal antibody (10309-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HeLa, cellules MCF-7, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10309-1-AP cible GPRC5A,RAI3 dans les applications de WB, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Chèvre, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPRC5A,RAI3 Protéine recombinante Ag0311
• Nom complet: G protein-coupled receptor, family C, group 5, member A
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC003665
• Symbole du gène: GPRC5A
• Identification du gène (NCBI): 9052
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPRC5A, also named as GPCR5A, RAI3 and RAIG1, belongs to the G-protein coupled receptor 3 family. It is an orphan G-protein coupled receptor (GPCR) that has been associated with malignancy and may play a role in the proliferation of breast cancer cells. GPRC5A is a potential molecular target for treatment of breast cancers. It is suppressed in some human lung carcinoma cells. (PMID:15788639, 20563252, 19552806 )

Publications

Species	Application	Title
goat	IHC	Cell Prolif; Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysis; Authors - Peng Lin
human	WB	Sci Total Environ; GPRC5A reduction contributes to pollutant benzo[a]pyrene injury via aggravating murine fibrosis, leading to poor prognosis of IIP patients.; Authors - Ziling Huang ; KO Validated
Human	WB	Biol Open; Quantitative proteomic profiling of tumor-associated vascular endothelial cells in colorectal cancer.; Authors - Guoqiang Wang
human	WB	Oncol Lett; Clinical significance of reduced GPRC5A expression in surgically resected non-small cell lung cancer.; Authors - Er Jin
human	WB	Exp Lung Res; GPRC5a suppresses the proliferation of non-small cell lung cancer under wild type p53 background.; Authors - Jin Er
		Mol Med Rep; Lung cancer suppressor gene GPRC5A mediates p53 activity in non‑small cell lung cancer cells in vitro.; Authors - Er Jin

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