Anticorps Polyclonal de lapin anti-GPT / ALT1

GPT / ALT1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 16897-1-AP

Synonymes

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ ALT1, GPT1

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Galerie de données de validation

• 

  WB analysis using 16897-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16897-1-AP (GPT/ ALT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat, tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below

Informations sur le produit

16897-1-AP cible GPT / ALT1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPT / ALT1 Protéine recombinante Ag10424
• Nom complet: glutamic-pyruvate transaminase (alanine aminotransferase)
• Masse moléculaire calculée: 496 aa, 55 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC018207
• Symbole du gène: ALT1
• Identification du gène (NCBI): 2875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

Publications

Species	Application	Title
		Cancer Discov; Selective Alanine Transporter Utilization Creates a Targetable Metabolic Niche in Pancreatic Cancer.; Authors - Seth J Parker
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
mouse	WB	Cell Rep; T Cell Activation Depends on Extracellular Alanine.; Authors - Noga Ron-Harel
human	WB	Oncotarget; Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.; Authors - Santosh K Bharti
human, mouse	WB	J Proteome Res; Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.; Authors - Shizhen Qin
mouse	WB	Mediators Inflamm; Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.; Authors - Shuaiwei Wang

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